Thursday

THANKSGIVING

Friday

Monday

Nov.28

DNA Extraction

DNA Extraction LAB

* DNA Extraction questions (per group)

*Homework: Recombinant DNA 1 (rDNA1): Read pages 264-267

1. What are restriction enzymes? Where do they naturally exist?

2. Figure page 265: Why did enzymes 1 and 2 result in different DNA lengths?

3. How does Electrophoresis separate DNA fragments from each other?

4. How can one tell if a restriction enzyme really cut a DNA molecule as expected? (see figure 9.3).

5. What are “Sticky ends”?

6. Go to p.277 Fig 9.11. Answer “Apply”?

Due: Wednesday

Tuesday

29

Staff

Application: Restriction, Transformation

* Paper Plasmid: Marking

Wednesday

30

Application: Restriction, Transformation

* Paper Plasmid: Cutting and pasting

* Analysis of paper plasmid activity (per group)

Thursday

Dec 1

Application: Electrophoresis and DNA fingerprinting

* Paper Plasmid – cont.

* Recombinant “Plasmid”

*Homework rDNA2: DNA Fingerprinting; pages 272-274

1. What are three uses for DNA fingerprinting (p.274 bottom)?

2. How does DNA fingerprinting work? (2 steps, p.267, 272)

3. Why do they choose to cut in ‘non-coding’ regions of the DNA (p.272)?

4. Why is it easier to prove innocence rather than that guilt? (p.274).

Due: Monday (postponed)

Friday

2

Application: DNA Fingerprinting

* Gel electrophoresis demonstration

* DNA Fingerprinting: reading from “DragonFly” Book

Monday

5

Application: DNA Fingerprinting

Application: GMOs

* Video: “Crime Seen”

* Video: “Harvest of Fear”

- Bt Corn

- Breeding vs. transgenics

- Environmental effects

* Discussion

* Videonotes: “Crime seen”

* Homework rDNA3: Genetic engineering p.275-279

1. What is “recombinant DNA”?

2. What is a transgenic organism?

3. What are plasmids? How are they useful for genetic engineering?

4. How does genetic engineering depend on a shared genetic code (=p.277 “Summarize”)

5. What are at least three uses for genetic engineering?

Due: Wednesday

* Videonotes: Harvest of Fear

Tuesday

6

Staff Development

Application: GMOs

* Video: “Harvest of Fear”

* Reading

* Discussion

Wednesday

7

Application: GMOs

* Video: Harvest of Fear –cont.

* Discussion

Homework:

FINAL Review Guide

Due: Final Exam

Thursday

8

Application: Bioinformatics

* Video:

- “Genetic modification”

Genomics, DNA sequencing.

Friday

9

Review: Vocabulary

Monday

12

Review: Biomolecules, Cells, Molecular Biology