Comments

• Jake

  RNAlater Tissue Collection: RNA Stabilization Solution is an
  aqueous tissue storage reagent that rapidly permeates most tissues
  to stabilize and protect RNA in fresh specimens. It eliminates
  the need to immediately process or freeze samples; the
  specimen can simply be submerged in RNAlater Solution and
  stored for analysis at a later date.
  Samples in RNAlater Solution can be stored for extended periods
  under conditions where RNA degradation would normally
  take place rapidly. Tissues can be stored indefinitely
  in RNAlater Solution at –20°C or below

  4/14/2009 3:17:09 PM
• Erica

  Small tissue samples are placed in the small bottles of RNA later solution (right) for stablizing and preservation purposes. It 'keeps' the RNA for up to a week. Once all the other equipment is ready to be used, the tissue is removed from the solution and used as needed - in our case, blended and centrifuged.

  4/14/2009 6:36:21 PM
• Matt

  RNA later, is used to keep the RNA stabalized. It protects the tissue enough so over a period of time it can still be used to harvest RNA. The temperature you want to store it at depends on the amount of time the tissue will be in the RNAlater.

  4/14/2009 10:59:27 PM
• Ariel

  You would collect the tissue from your sample specimen and submerged in RNAlater in order to protect the RNA in the sample fresh for up to a week.

  4/15/2009 5:16:23 PM
• Emily

  First, you would take a small amount of RNAlater and pour it into the small container. Then, take a small tissue sample and place it in the containter. This will protect and preserve the RNA sample for up to about a week at room temperature. This prevents the RNA from decaying so you can run testing on the RNA later without having to freeze it, or perform the experiements immediately.

  4/15/2009 5:40:36 PM
• JeannieLong

  Good Work AP Biology II Honors students!

  4/16/2009 5:05:57 PM
• JeannieLong

  Jake. Good work finding information. However, you did not cite your source. Please do so (in correct format). If you cited word for word (and it looks like you did), resubmit using quotations.
  Thank you,
  Mrs. Long

  4/16/2009 5:36:05 PM
• Matt

  RNA later, is used to keep the RNA stabalized. It protects the tissue enough so over a period of time it can still be used to harvest RNA. The temperature you want to store it at depends on the amount of time the tissue will be in the RNAlater.
  
  Ambion. RNA Extraction Project. 2009. 14 April 2009.<http://www.ambion.com/catalog/CatNum.php?7021>

  4/20/2009 9:15:59 PM
• Kristen

  RNAlater is used to keep the removed tissue fresh and stable until it is needed for an experiment. To use it, one would submerge the specimen in the liquid and seal the container. At room temperature it can be kept for up to a week, but if cooled or frozen it will last longer.

  4/26/2009 6:39:02 PM
• Jake

  RNAlater Tissue Collection: RNA Stabilization Solution is an
  aqueous tissue storage reagent that rapidly permeates most tissues
  to stabilize and protect RNA in fresh specimens. It eliminates
  the need to immediately process or freeze samples; the
  specimen can simply be submerged in RNAlater Solution and
  stored for analysis at a later date.
  Samples in RNAlater Solution can be stored for extended periods
  under conditions where RNA degradation would normally
  take place rapidly. Tissues can be stored indefinitely
  in RNAlater Solution at –20°C or below (http://www.biocompare.com/ProductReview.aspx?r=182). sorry :)

  4/26/2009 8:31:24 PM
• Jake

  I accidentally cited the wrong website. Here is the real one that I got my information off of. (http://www.ambion.com/techlib/prot/bp_7020.pdf)

  4/26/2009 8:33:28 PM
• JeannieLong

  Thanks for citing your reference Jake - always essential!

  4/28/2009 7:32:03 PM
• Amalia

  Tissue extracted from an organism can be put into the RNAlater and can be stored for up to a week. It preserves the RNA so that extraction is possible later on.

  5/13/2009 10:15:20 PM
• Shaina

  This solution, RNAlater, is aqueous, is nontoxic, and serves to store tissues for later use or testing. It is able to save the tissue by stabilizing and preventining any decompsotion of the cellular RNA. To use RNAlater, the tissue sample is placed in a small container and then the desired amount of solution is placed within the container.
  
  
  Invitrogen. "Make Time Stand with RNAlater Solution." 2009. Invitrogen Cooperation. 14 May 2008. <www.invitrogen.com/.../rnalater.html>.

  5/14/2009 11:06:28 AM
• jennifer

  RNA later protects the tissue sample and holds it for up to a week at room temperature. Samples can be harvested for longer periods of time if kept at colder temperatures (usually frozen or refrigerated, depending on the time period.) The samples are kept stable in this solution until the RNA extractions are made by the students.

  5/14/2009 1:52:23 PM
• Zach

  RNA later protects the different tissues you put in it. Scientists use this solution to keep the samples preserved for a period of time when they aren't using them.

  5/20/2009 10:13:23 PM
• Sofia

  RNA later tissue collection is used to store fresh RNA tissue to be used later. It ensures that the RNA is still fresh if you would like to use at a later time after collection the RNA. RNA later solution can be stored at room temperature and can preserve the RNA for up to a week. It is very convenient because it eliminates the need to process it immediately or to freeze the sample. In addition, it also stores the sample without harming the quality or the amount of the RNA collected from the isolation.

  5/25/2009 5:41:06 PM
• Rebecca

  This product is used when storing tissue samples to be used for RNA extraction. It allows users to isolate the RNA for time periods up to a month when stored at 4C or for an even longer, indefinate period of time when stored at -20C without the substance losing its integrity. This substance is particularly helpful because it allows the user to avoid the dangers associated with the use of liquid nitrogen.
  
  "Ambion's RNAlater solution." Biocompare. 25 May 2004. 26 May 2009 <"www.biocompare.com/ProductReview.aspx

  5/26/2009 7:06:01 PM
• Lauren

  RNA later is what keeps the dna preserved up to a week at room temperature. This allows you to take more than one day to perform an experiment.

  5/27/2009 10:46:12 PM